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This section is written in partnership with UR Vistahermosa experts from Spain.

DNA fragmentation in sperm

The initial assessment of infertile couples must include a semen analysis, since the male factor accounts for half of infertility cases. A semen analysis looks mainly at the macroscopic, microscopic and biochemical characteristics of the ejaculate in accordance with World Health Organization recommendations.

The total number of sperm in the semen is a reflection of their production in the testicles and the permeability of the post-testicular ducts. The microscopic features analysed include the concentration, motility and morphology of the sperm. Nonetheless, none of these parameters is, on its own, a powerful predictor of infertility.

Efforts to find the ideal marker to differentiate infertile men from fertile men and to predict the possibility of achieving pregnancy have currently intensified.

Despite this, the cause of infertility cannot be determined in 30-50% of infertile males, who are classified as men with idiopathic infertility. Clinical evidence in assisted reproduction suggests that failure in fertilisation or embryo development may be the result of an alteration in the sperm components mentioned above. However, these elements are not assessed in the conventional semen analysis. Sperm that are damaged or have DNA fragmentation are able to fertilise, and that damage may persist even after fertilisation takes place.

During the in-vivo fertilisation process, the female reproductive apparatus has mechanisms against the selection of DNA-damaged sperm. This feature does not exist with in vitro fertilisation (IVF) or intracytoplasmic sperm injection.

It has been proposed that the main origin of the f ragmentation of sperm DNA is oxidative stress. This molecular event occurs when there is an imbalance between the formation of reactive oxygen species and the antioxidant defence. Several research projects have shown the relationship between a lower antioxidant capacity and high levels of oxidative stress in semen samples. In addition, other studies point to this relationship with DNA-damaged sperm.

The importance of semen analysis

This examination consists of finding the breaks or injuries in the genetic material of the sperm. The higher the number of injuries, the lower the integrity of the genetic material, thus reducing the pregnancy rate.

Scientific evidence points to the fact that the fragmentation of sperm DNA has a significant impact on natural and assisted reproduction. There is also a strong association between the fragmentation of sperm DNA and early pregnancy loss. Therefore, the analysis of the integrity of sperm DNA should be included in the male fertility assessment protocol.

The sperm DNA fragmentation test is recommended in the following cases:

  • Infertility of unknown (idiopathic) cause.
  • After repeated failures in assisted reproduction techniques
  • Cases where poor embryo quality has been observed
  • Patients who have suffered repeated miscarriages.
  • Varicocele.
  • Cases of frozen semen (a check is made to see whether the frozen sample has acceptable levels of fragmentation)
  • Episodes of fever in the past three months.

With regard to assisted reproduction treatments, it has been shown that sperm capacitation techniques – especially density gradients – are effective in elimination many of the DNA-damaged sperm.

If the fragmentation index is low, the egg may be able to repair this damage on its own after fertilisation. We do not have a clear understanding of this mechanism, but it does depend largely on the type of injuries and on the quality of the egg.

Some antioxidants that improve semen quality include vitamins C, E and A. These are naturally found in fruits, vegetables, vegetable oils, brown rice, soybeans, chocolate, oregano, tea, etc. Treatment with antioxidants for two or three months may reduce the fragmentation of sperm DNA by up to 20%.

Article source:
This article has been published in Creando Familias magazine by UR Vistahermosa clinic from Spain.

Arlén Hernández
Arlén Hernández
Embryologist, UR Managua

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